Indeever M. 2023 | BASIS Independent Silicon Valley
- Project Title: Sequencing the Transcriptome of the Freshwater Angelfish
- Basis Independent Advisor: Dr. Karen Allendoerfer
- Internship Location: BioCurious
- Onsite Mentor: Mr. Johan Sosa
The freshwater angelfish is a popular cichlid fish kept in the aquarium hobby. Native to South America, it is well known for its thorough parental care of offspring and monogamous breeding patterns. The freshwater angelfish could be a potential model organism for future biomedical research, as cichlids have a similar developmental pattern and cellular biology to humans. But for such a reality to occur, a thorough understanding of the genetics of the angelfish is required. In a previous study, I assembled the first-ever complete genome of the freshwater angelfish with the affordable Nanopore MinION sequencing technology. The genome was 735 Megabases in size and included over 24,000 protein-coding sequences. Now that a complete DNA genome of the angelfish has been assembled, the next step is to perform whole RNA transcriptome sequencing of the angelfish with the aforementioned Nanopore technology. It’s expected that if RNA from the angelfish is extracted and sequenced, then essential pieces of information will be revealed, including transcript abundance, genetic networks, and coding and non-coding transcripts. With this information, a more thorough understanding of the biology of the angelfish will be gained, and a future where the angelfish is used as a model organism may become a reality. This research will be conducted at BioCurious, a local community biology lab for citizen scientists.