Week 8: Is It Pronounced Data Or Data?

Well. Let’s jump right into it! 

Let’s review the amount of data I was able to collect with 62 hours of sequencing! 

As seen above, within 62 hours, I was able to obtain 17 million sequencing reads totaling 15.72 Gigabases!! Around 80% of these reads were above a quality score of 9 which corresponds to approx 90% raw accuracy.

The N50 length of the cDNA transcripts was 1.32 kilobases in length and the distribution of read lengths can be seen above. Clearly, we were able to get the long reads nanopore is well known for. For comparison, traditional transcriptome sequencing platforms fragment cDNA reads into fragments around 75-101 bp in length. My reads were over 10 times as long!

The Pore activity graph above shows that around 70% of the nanopores were actively sequencing, with that number steadily decreasing as the sequencing run progressed. This indicates that the pores are of good quality, and that I loaded sufficient library into the sequencing device. 

Finally, the translocation speed (or sequencing speed) of the individual nanopores remained around 400 bp/s throughout the run which is ideal. 

So far, the sequencing has been successful and we have been able to collect a significant amount of data. Come back to my next post where we will begin discussing data analysis! Thanks again for reading!